Rapid and Quantitative Detection of Lung Cancer Biomarker ENOX2 Using a Novel Aptamer in an Electrochemical DNA-Based (E-DNA) Biosensor

To overcome early cancer detection challenges, diagnostic tools enabling more sensitive, rapid, and noninvasive are necessary. An attractive target for blood tests is human Ecto-NOX disulfide–thiol exchanger 2 (ENOX2), expressed in most types regularly shed into sera. Here, we developed an electrochemical DNA-based (E-DNA) biosensor that rapidly detects physiologically relevant levels of ENOX2. identify ENOX2-binding aptamers could potentially be used a biosensor, recombinantly ENOX2 was as binding oligonucleotide library pull-down generated highly enriched aptamer. This candidate aptamer sensitively bound via gel mobility shift assays. enable this to function E-DNA the sequence modified adopt two conformations, one capable binding, with disrupted binding. Upon introduction, conformational state resulted changed dynamics redox reporter molecule, which significant, target-specific electrical current readout change. sensitivity at or below range. The design presented here may development detection.